Detailed Reaction Mechanism of Macrophomate Synthase
نویسندگان
چکیده
منابع مشابه
Detailed reaction mechanism of macrophomate synthase. Extraordinary enzyme catalyzing five-step transformation from 2-pyrones to benzoates.
Macrophomate synthase from the fungus Macrophoma commelinae IFO 9570 is a Mg(II)-dependent dimeric enzyme that catalyzes an extraordinary, complex five-step chemical transformation from 2-pyrone and oxalacetate to benzoate involving decarboxylation, C-C bond formation, and dehydration. The catalytic mechanism of the whole pathway was investigated in three separate chemical steps. In the first d...
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mation to modify an existing enzyme for adcling another function or to create a new enzyme that can transform molecules in a stereoeontrolled manner, Yamamoto's group4'5} have previously shown that macrophomic acid (1)6] and the phytotoxin, pyrenochaetic acid (2),7) were biotransformed from the corresponding 2-pyrones, 3 and 4 (pyrenocine A8,9)), and a C3-acid precursor by the fungus, Macrophom...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 2000
ISSN: 0021-9258
DOI: 10.1074/jbc.m003119200